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(BIO 281) Bacterial Transformation Lab Report

Sandra Watson
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Arizona State University


Conceptual Approaches to Biology for Majors I (BIO 281)

Nguyen 2
The technique of transforming cells such as bacteria in genetic is pertinent for the
improvements of molecular biology. The objectives of this lab are: to perform genetic
transformation using the bacterial transformation method, and to observe the results of bacterial
transformation in various growth condition. As mentioned above, the methodology used was
bacterial transformation protocol. Which is a series of technique used to introduce a foreign
plasmid into the bacteria (E. Coli), then the bacteria will multiply within the plasmid.
If executed properly, the results should display growth and glow under –pGLO with LB, +pGLO
with LB/Amp, +pGLO with LB/Ara, and +pGLO with LB/Ara/Amp. Therefore, leads to the
conclusion that bacteria will transform under the right conditions.
The bacteria being transformed in this lab is Escherichia Coli (E. Coli), which is a rodshaped
bacteria that is usually found in the lower intestine of warm-blooded organisms. The
process of bacterial transformation revolves mainly around the plasmid needed for bacteria’s
growth. Within the plasmid, there are genes such as: the AraC protein which will regulate the Larabinose
operons in E.



(BIO 281) Bacterial Transformation Lab Report

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