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General Chemistry (CH 221 Lab) Beer's Law Lab Report

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Sandra Watson
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Portland State University

Lab for General Chemistry (CH 221 Lab)

Determining the Concentration of a Solution: Beer’s Law
Abstract:
The task for this lab was to prepare Allura Red standard solutions and using colorimeter to measure
the absorbance of each of them. The other task was to find the relationship between absorbance and
concentration of a solution and using that relationship to determine the concentration of Allura Red
in commercially available beverage (in this case red Gatorade). The result of the experiment showed
that the concentration of Allura Red in red Gatorade is 3.88*10^-6 M.
Introduction:
The task for this lab was to prepare five solutions of Allura Red with different concentrations. The
next step was to determine the concentration of each solution. After that, each standard solution was
transferred to a small, rectangular cuvette that is placed into the colorimeter. The amount of light
that passes through the solution and strikes the photocell was used to compute the absorbance of
each solution. After the absorbance of each solution was known, it was used to make a graph of
absorbance vs. concentration. Next step was to find the equation of the graph, namely calibration
curve. Calibration curve is a general method for determining the concentration of a substance in an
unknown sample by comparing the unkown to a set of standard samples of known concentration.
From the graph, the linear realtionship between absorbance and concentration for a solution was
determined. That relationship is also known as Beer’s law.

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 General Chemistry (CH 221 Lab) Beer's Law Lab Report

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