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General Microbiology (BIOS 350) DATA Sheet Questions Micro

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Sandra Watson
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University of Illinois at Chicago

General Microbiology (BIOS 350)

1 In your own words, what is the application (purpose) of MSA?

2 Which ingredient(s) in MSA supply(ies)

3 Is MSA a defined or an undefined medium? Provide the reasoning behind your choice and
explain why this formulation is desirable.

4 In our own words, what is the role of sodium chloride in MSA and how does it work?

5 In your own words, what are the roles of mannitol and phenol red in MSA?

6 Think about the results you recorded.
a. Growth on the MSA and NA plates was recorded as “good growth,” “poor growth,” or “no
growth.” These are qualitative and, at least for the first two, subjective terms. What did you
use to establish what constituted “good growth?”

b. Why wouldn’t it be advisable to compare growth of the organisms on each plate to each
other? There are at least two answers to this question!

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General Microbiology (BIOS 350) DATA Sheet Questions Micro

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