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Microbiology (BIOL 3880) Gram Stain Lab Report

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Lab Report 1 – Grade: C+ – Introduction To Simple Stain And Gram Stain

Lab report


Georgia State University


Microbiology (BIOL 3880)

Nerixa Portillo
BIOL 2310
September 17, 2017
Introduction to Simple Stain and Gram Stain
The study of Microbiology is small organisms, but the naked eye cannot see those small
organisms. Staining is necessary for Microbiology to distinguish the structure of microbes
because they are translucent, light passes through them, and that is why we cannot see it with our
own eyes. (Maxwell 2017) There are two major stains they are Simple Stain and Differential
Stains, in other words, Gram Stain. There are two categories of stains Acidic and Basic dye.
Acidic dye is a negative charge, and Basic dye is a positive charge. The difference between
simple stain and gram stain is that simple stain has one simple stain and gram stain have more
steps for staining because of the structure of bacteria’s cell wall. The simple stain contains a
basic dye which contains methyl blue, crystal violet, safranin, and malachite green. Though
acidic dyes do not stain the cells; they stain the background because the cells repel the negative
charge dye. The purpose of simple stain is to visualize the structures of bacteria. Gram stain is a
complex stain, the reason for gram stain is because cells of different species have different
physical and chemical properties. (Maxwell 2017) It is also a used procedure for staining
bacteria. Gram Stain is mostly in use in the clinical setting and research laboratories.



Microbiology (BIOL 3880) Gram Stain Lab Report

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